Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM).

ABSTRACT:

Introduction

Safranal is an active component of the traditional Tibetan medicine (TTM) saffron, which has potential anticancer activity.

Methods and results

Here, we studied the therapeutic effect and mechanism of safranal on GBM. CCK-8, GBM-brain organoid coculture experiments and 3D tumour spheroid invasion assays showed that safranal inhibited GBM cell proliferation and invasion in vitro. Network pharmacology, RNA-seq, molecular docking analysis, western blotting, apoptosis, and cell cycle assays predicted and verified that safranal could promote GBM cell apoptosis and G2/M phase arrest and inhibit the PI3K/AKT/mTOR axis. In vivo experiments showed that safranal could inhibit GBM cell growth alone and in combination with TMZ.

Conclusion

This study rev

SUBMITTER: Yang X 

PROVIDER: S-EPMC10509474 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature