Modified Ion Source for the Improved Collisional Activation of Protein Complexes.

ABSTRACT: The analysis of large molecules is challenging, as they often have salts and adducts retained through the electrospray process, which increase the observed mass and compromise the achievable mass resolution. Mild collisional activation has been shown to be very effective for the removal of adducts and increases both measurement accuracy and mass resolution of large (>100 kDa) protein complexes. Collisionally activated protein ions are more completely desolvated due to the increased number of collisions when trapped following activation. A short square quadrupole maintained at 300 mTorr by a mechanical pump was added between the ion funnel and transmission quadrupole. This configuration and operation effectively removed adducts from the 800 kDa tetradecamer GroEL as well as fragmented small

SUBMITTER: Schrader RL 

PROVIDER: S-EPMC10510017 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature