First isolation of Rickettsia amblyommatis from Amblyomma mixtum in Colombia.

ABSTRACT:

Background

Rickettsiae are obligate intracellular Gram-negative bacteria that are the causative agent of rickettsioses and are spread to vertebrate hosts by arthropods. There are no previous reports of isolation of Rickettsia amblyommatis for Colombia.

Methods

A convenience sampling was executed in three departments in Colombia for direct collection of adult ticks on domestic animals or over vegetation. Ticks were screened for the presence of Rickettsia spp. by real-time polymerase chain reaction (qPCR) amplifying the citrate synthase gene (gltA), and the positive sample was processed for isolation and further molecular characterization by conventional PCR. The absolute and relative frequencies were calculated for several tick species variables. All products from conventiona

SUBMITTER: Chaparro-Gutierrez JJ 

PROVIDER: S-EPMC10510177 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature