CDC42-mediated Wnt signaling facilitates odontogenic differentiation of DPCs during tooth root elongation.

ABSTRACT:

Background

CDC42 is a member of Rho GTPase family, acting as a molecular switch to regulate cytoskeleton organization and junction maturation of epithelium in organ development. Tooth root pattern is a highly complicated and dynamic process that dependens on interaction of epithelium and mesenchyme. However, there is a lack of understanding of the role of CDC42 during tooth root elongation.

Methods

The dynamic expression of CDC42 was traced during tooth development through immunofluorescence staining. Then we constructed a model of lentivirus or inhibitor mediated Cdc42 knockdown in Herwig's epithelial root sheath (HERS) cells and dental papilla cells (DPCs), respectively. Long-term influence of CDC42 abnormality was assessed via renal capsule transplantation and in situ inj

SUBMITTER: Zhou T 

PROVIDER: S-EPMC10510226 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature