Expansion of human bone marrow-derived mesenchymal stromal cells with enhanced immunomodulatory properties.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) have broad potential as a cell therapy including for the treatment of drug-resistant inflammatory conditions with abnormal T cell proliferation such as graft-versus-host disease (GVHD). Clinical success, however, has been complicated by the heterogeneity of culture-expanded MSCs as well as donor variability. Here, we devise culture conditions that promote expansion of MSCs with enhanced immunomodulatory functions both in vitro and in animal models of GVHD.

Methods

Human bone marrow-derived MSCs were expanded at high-confluency (MSC_HC) and low-confluency state (MSC_LC). Their immunomodulatory properties were evaluated with in vitro co-culture assays based on suppression of activated T cell proliferation and secr

SUBMITTER: Neo SH 

PROVIDER: S-EPMC10510228 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature