Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice.

ABSTRACT:

Background

Mechanical ventilation is a supportive therapy used to maintain respiratory function in several clinical and surgical cases but is always accompanied by lung injury risk due to improper treatment. We investigated how tidal volume and oxygen delivery would contribute independently or synergistically to ventilator-induced lung injury (VILI).

Methods

Under general anesthesia and tracheal intubation, healthy female C57BL/6 N mice (9 weeks old) were randomly ventilated for 2 h by standard (7 ml/kg) or high (14 ml/kg) tidal volume at positive end-expiratory pressure (PEEP) of 2 cmH₂O, with room air, 50% O₂ (moderate hyperoxia), or 100% O₂ (severe hyperoxia); respectively. Mice were sacrificed 4 h after mechanical ventilation, and lung ti

SUBMITTER: Xu Y 

PROVIDER: S-EPMC10510264 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature