Dual inhibition of glycolysis and glutaminolysis for synergistic therapy of rheumatoid arthritis.

ABSTRACT:

Background

Synovial fibroblasts in rheumatoid arthritis (RAFLS) exhibit a pathological aberration of glycolysis and glutaminolysis. Henceforth, we aimed to investigate if dual inhibition of these pathways by phytobiological compound c28MS has the potential of synergistic therapy for arthritis by targeting both glucose and glutamine metabolism.

Methods

The presence of HK2 and GLS across various cell types and associated gene expression in human synovial cells and a murine model of arthritis was evaluated by scRNA-seq. The metabolic profiling of RAFLS cells was done using H¹-nuclear magnetic resonance spectroscopy under glycolytic and glutaminolytic inhibitory conditions by incubating with 3-bromopyruvate, CB839, or dual inhibitor c28MS. FLS functional analysis was

SUBMITTER: Ahmed S 

PROVIDER: S-EPMC10510293 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature