High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis.

ABSTRACT:

Background

The aim of study was to observe the effect of increased lactate levels during high-intensity interval training (HIIT) on protein lactylation, identify the target protein, and investigate the regulatory effect of lactylation on the function of the protein.

Methods

C57B/L6 mice were divided into 3 groups: the control group, HIIT group, and dichloroacetate injection + HIIT group (DCA + HIIT). The HIIT and DCA + HIIT groups underwent 8 weeks of HIIT treatment, and the DCA + HIIT group was injected DCA before HIIT treatment. The expression of lipid metabolism-related genes was determined. Protein lactylation in subcutaneous adipose tissue was identified and analyzed using 4D label-free lactylation quantitative proteomics and bioinformatics analyses. The fatty acid synt

SUBMITTER: Chen X 

PROVIDER: S-EPMC10510295 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature