Engineered In Vitro Tumor Model Recapitulates Molecular Signatures of Invasion in Glioblastoma.

ABSTRACT: Glioblastoma stem cells (GSCs) play an important role in the invasive nature of glioblastoma (GBM); yet, the mechanisms driving this behavior are poorly understood. To recapitulate tumor invasion in vitro, we developed a GBM tumor-mimetic hydrogel using extracellular matrix components upregulated in patients. We show that our hydrogel facilitates the infiltration of a subset of patient-derived GSCs, differentiating samples based on phenotypic invasion. Invasive GSCs are enriched for injury-responsive pathways while noninvasive GSCs are enriched for developmental pathways, reflecting established GSC stratifications. Using small molecule inhibitors, we demonstrate that the suppression of matrix metalloprotease and rho-associated protein kinase processes results in a significant reduction of

SUBMITTER: Smith LJ 

PROVIDER: S-EPMC10510514 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature