Scalable Production of Peptides for Enhanced Struvite Formation via Expression on the Surface of Genetically Engineered Microbes.

ABSTRACT: A promising method for recycling phosphate from wastewater is through precipitation of struvite (MgNH₄PO₄·6H₂O), a slow-release fertilizer. Peptides have been shown to increase the yield of struvite formation, but producing peptides via solid phase synthesis is cost prohibitive. This work investigates the effects of peptide-expressing bacteria on struvite precipitation to provide a sustainable and cost-efficient means to enhance struvite precipitation. A peptide known for increased struvite yield was expressed on a membrane protein in Escherichia coli(E. coli), and then 5 mL precipitation reactions were performed in 50 mL culture tubes for at least 15 min. The yield of struvite crystals was examined, with the presence of peptide-expressing

SUBMITTER: Hostert JD 

PROVIDER: S-EPMC10510520 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature