Dual Input-Controlled Synthetic mRNA Circuit for Bidirectional Protein Expression Regulation.

ABSTRACT: Synthetic mRNA circuits manipulate cell fate by controlling output protein expression via cell-specific input molecule detection. Most current circuits either repress or enhance output production upon input binding. Such binary input-output mechanisms restrict the fine-tuning of protein expression to control complex cellular events. Here we designed mRNA circuits using enhancer/repressor modules that were independently controlled by different input molecules, resulting in bidirectional output regulation; the maximal enhancement over maximal repression was 57 fold. The circuit either enhances or represses protein production in different cells based on the difference in the expression of two microRNAs. This study examined novel bidirectional circuit designs capable of fine-tuning protein pro

SUBMITTER: Tan K 

PROVIDER: S-EPMC10510700 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature