Cardiopulmonary bypass in a rat model may shorten the lifespan of stored red blood cells by activating caspase-3.

ABSTRACT:

Background

Red blood cell transfusion is required for many types of surgery against cardiovascular disease, and the function of transfused cells appears to decline over time. The present study examined whether transfusion also reduces red blood cell lifespan in a rat model.

Material and methods

Bypass in rats were established by connecting a roll pump to the femoral artery and vein. Then FITC-labeled stored red blood cells from rats were transfused in the animals, and the cells in circulation were counted after transfusion. In separate experiments, stored red blood cells were incubated with bypass plasma in vitro, and the effects of incubation were assessed on cell morphology, redox activity, ATP level, caspase-3 activity, and phosphatidylserine exposure on the cell surface.

SUBMITTER: Han L 

PROVIDER: S-EPMC10511131 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature