Single molecule analysis of CENP-A chromatin by high-speed atomic force microscopy.

ABSTRACT: Chromatin accessibility is modulated in a variety of ways to create open and closed chromatin states, both of which are critical for eukaryotic gene regulation. At the single molecule level, how accessibility is regulated of the chromatin fiber composed of canonical or variant nucleosomes is a fundamental question in the field. Here, we developed a single-molecule tracking method where we could analyze thousands of canonical H3 and centromeric variant nucleosomes imaged by high-speed atomic force microscopy. This approach allowed us to investigate how changes in nucleosome dynamics in vitro inform us about transcriptional potential in vivo. By high-speed atomic force microscopy, we tracked chromatin dynamics in real time and determined the mean square displacement and diffusion constant fo

SUBMITTER: Melters DP 

PROVIDER: S-EPMC10511241 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature