Identification of single nucleotide polymorphisms (SNPs) associated with chronic graft-versus-host disease in patients undergoing allogeneic hematopoietic cell transplantation.

ABSTRACT:

Introduction

Chronic graft-versus-host disease (cGVHD) is a debilitating side effect of allogeneic hematopoietic cell transplantation (HCT), affecting the quality of life of patients. We used whole exome sequencing to identify candidate SNPs and complete a multi-marker gene-level analysis using a cohort of cGVHD( +) (N = 16) and cGVHD( -) (N = 66) HCT patients.

Methods

Saliva samples were collected from HCT patients (N = 82) pre-conditioning in a multi-center study from March 2011 to May 2018. Exome sequencing was performed and FASTQ files were processed for sequence alignments. Significant SNPs were identified by logistic regression using PLINK2_v3.7 and Fisher's exact test. One cGVHD( -) patient sample was excluded from further analysis since no SNP was present i

SUBMITTER: Mougeot JC 

PROVIDER: S-EPMC10511391 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature