Partial skeletal muscle-specific Drp1 knockout enhances insulin sensitivity in diet-induced obese mice, but not in lean mice.

ABSTRACT:

Objective

Dynamin-related protein 1 (Drp1) is the key regulator of mitochondrial fission. We and others have reported a strong correlation between enhanced Drp1 activity and impaired skeletal muscle insulin sensitivity. This study aimed to determine whether Drp1 directly regulates skeletal muscle insulin sensitivity and whole-body glucose homeostasis.

Methods

We employed tamoxifen-inducible skeletal muscle-specific heterozygous Drp1 knockout mice (mDrp1^+/-). Male mDrp1^+/- and wildtype (WT) mice were fed with either a high-fat diet (HFD) or low-fat diet (LFD) for four weeks, followed by tamoxifen injections for five consecutive days, and remained on their respective diet for another four weeks. In addition, we used primary human skeletal muscle cells (HS

SUBMITTER: Kugler BA 

PROVIDER: S-EPMC10511484 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature