Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures.

ABSTRACT: Impaired function of the retinal pigment epithelium (RPE) resembles a hallmark in many retinal diseases; thus, co-cultivation models with RPE and retinal explants are useful to investigate these. Here, we present an easy-to-handle direct co-cultivation protocol, containing a functional, primary RPE monolayer and retinal explants. We describe in detail steps for establishing the monolayer and the direct co-cultivation. Techniques, which allow users to investigate the integrity and functionality of the RPE monolayer, are presented and the co-cultivation was tested under stress conditions.

SUBMITTER: Fietz A 

PROVIDER: S-EPMC10511932 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature