Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis.

ABSTRACT: Ustilago maydis is a biotrophic phytopathogenic fungus that causes corn smut disease. As a well-established model system, U. maydis is genetically fully accessible with large omics datasets available and subject to various biological questions ranging from DNA-repair, RNA-transport, and protein secretion to disease biology. For many genetic approaches, tight control of transgene regulation is important. Here we established an optimised version of the Tetracycline-ON (TetON) system for U. maydis. We demonstrate the Tetracycline concentration-dependent expression of fluorescent protein transgenes and the system's suitability for the induced expression of the toxic protein BCL2 Associated X-1 (Bax1). The Golden Gate compatible vector system contains a native minima

SUBMITTER: Ingole KD 

PROVIDER: S-EPMC10512375 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature