Long-read sequencing reveals the landscape of aberrant alternative splicing and novel therapeutic target in colorectal cancer.

ABSTRACT:

Background

Alternative splicing complexity plays a vital role in carcinogenesis and cancer progression. Improved understanding of novel splicing events and the underlying regulatory mechanisms may contribute new insights into developing new therapeutic strategies for colorectal cancer (CRC).

Methods

Here, we combined long-read sequencing technology with short-read RNA-seq methods to investigate the transcriptome complexity in CRC. By using experiment assays, we explored the function of newly identified splicing isoform TIMP1 Δ4-5. Moreover, a CRISPR/dCasRx-based strategy to induce the TIMP1 exon 4-5 exclusion was introduced to inhibit neoplasm growth.

Results

A total of 90,703 transcripts were identified, of which > 62% were novel compared with current transcriptome a

SUBMITTER: Sun Q 

PROVIDER: S-EPMC10512518 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature