Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial-mesenchymal transition in lung endothelial cells.

ABSTRACT:

Background

Nestin, an intermediate filament protein, participates in various pathophysiological processes, including wound healing, angiogenesis, endothelial-mesenchymal transition (EndoMT), and fibrosis. However, the pathophysiological roles of lung nestin-expressing cells remain unclear due to conflicting reports. The objective of this study is to elucidate the characteristics and functions of lung nestin-expressing cells.

Methods

We conducted a series of in vitro and in vivo experiments using endothelial cell line MS1 and nestin-GFP mice. This animal model allows for nestin-expressing cell detection without the use of anti-nestin antibodies.

Results

Lung nestin-expressing cells occurred in approximately 0.2% of CD45^- cells and was co-expressed with epith

SUBMITTER: Daido W 

PROVIDER: S-EPMC10512559 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature