Absolute Quantification of Photoreceptor Outer Segment Proteins.

ABSTRACT: Photoreceptor cells generate neuronal signals in response to capturing light. This process, called phototransduction, takes place in a highly specialized outer segment organelle. There are significant discrepancies in the reported amounts of many proteins supporting this process, particularly those of low abundance, which limits our understanding of their molecular organization and function. In this study, we used quantitative mass spectrometry to simultaneously determine the abundances of 20 key structural and functional proteins residing in mouse rod outer segments. We computed the absolute number of molecules of each protein residing within an individual outer segment and the molar ratio among all 20 proteins. The molar ratios of proteins comprising three well-characterized constitutive

SUBMITTER: Skiba NP 

PROVIDER: S-EPMC10513726 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature