Phenotypic assay for cytotoxicity assessment of Balamuthia mandrillaris against human neurospheroids.

ABSTRACT:

Introduction

The phenotypic screening of drugs against Balamuthia mandrillaris, a neuropathogenic amoeba, involves two simultaneous phases: an initial step to test amoebicidal activity followed by an assay for cytotoxicity to host cells. The emergence of three-dimensional (3D) cell cultures has provided a more physiologically relevant model than traditional 2D cell culture for studying the pathogenicity of B. mandrillaris. However, the measurement of ATP, a critical indicator of cell viability, is complicated by the overgrowth of B. mandrillaris in coculture with host cells during drug screening, making it challenging to differentiate between amoebicidal activity and drug toxicity to human cells.

Methods

To address this limitation, we introduce a novel a

SUBMITTER: Whangviboonkij N 

PROVIDER: S-EPMC10513763 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature