Ontology highlight
ABSTRACT:
SUBMITTER: Cheng K
PROVIDER: S-EPMC10514076 | biostudies-literature | 2023 Dec
REPOSITORIES: biostudies-literature
Cheng Kai K Li Cao C Jin Jiachuan J Qian Xuezhen X Guo Jiayin J Shen Limini L Dai YiChen Y Zhang Xue X Li Zhanwei Z Guan Yichun Y Zhou Fei F Tang Jin J Zhang Jun J Shen Bin B Lou Xin X
Molecular therapy. Nucleic acids 20230909
Double-stranded DNA-specific cytidine deaminase (DddA) base editors hold great promise for applications in bio-medical research, medicine, and biotechnology. Strict sequence preference on spacing region presents a challenge for DddA editors to reach their full potential. To overcome this sequence-context constraint, we analyzed a protein dataset and identified a novel DddA<sub>tox</sub> homolog from <i>Ruminococcus</i> sp. <i>AF17-6</i> (RsDddA). We engineered RsDddA for mitochondrial base editi ...[more]