Ang II Controls the Expression of Mapkap1 by miR-375 and Affects the Function of Islet β Cells.

ABSTRACT:

Background

The RAS system is involved in the regulation of islet function, but its regulation remains unclear.

Objective

This study investigates the role of an islet-specific miR-375 in the effect of RAS system on islet β-cells.

Methods

miR-375 mimics and inhibitors were transfected into insulin-secreting MIN6 cells in the presence or absence of RAS component.

Results

Compared to control, in Ang II-treated MIN6 cells, miR-375 mimic transfection results in a decrement in cell viability and Akt-Ser levels (0.739±0.05 vs. 0.883±0.06 and 0.40±0.04 vs. 0.79±0.04, respectively), while the opposite occurred in miR-375 inhibitor-transfected cells (1.032±0.11 vs. 0.883±0.06 and 0.98±0.05 vs. 0.79±0.04, respectively, P<0.05). Mechanistically, transfection of miR- 375 mim

SUBMITTER: Lin X 

PROVIDER: S-EPMC10514520 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature