Osteopontin promotes gastric cancer progression via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway.

ABSTRACT:

Background

Gastric cancer (GC) is one of the most common malignant tumors. Osteopontin (OPN) is thought to be closely related to the occurrence, metastasis and prognosis of many types of tumors.

Aim

To investigate the effects of OPN on the proliferation, invasion and migration of GC cells and its possible mechanism.

Methods

The mRNA and protein expression of OPN in the GC cells were analyzed by real-time quantitative-reverse transcription polymerase chain reaction and western blotting, and observe the effect of varying degree expression OPN on the proliferation and other behaviors of GC. Next, the effects of OPN knockdown on GC cells migration and invasion were examined. The short hairpin RNA (shRNA) and negative control shRNA targeting OPN-shRNA were transfected into

SUBMITTER: Qin YC 

PROVIDER: S-EPMC10514723 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature