Profiling the polyadenylated transcriptome of extracellular vesicles with long-read nanopore sequencing.

ABSTRACT:

Background

While numerous studies have described the transcriptomes of extracellular vesicles (EVs) in different cellular contexts, these efforts have typically relied on sequencing methods requiring RNA fragmentation, which limits interpretations on the integrity and isoform diversity of EV-targeted RNA populations. It has been assumed that mRNA signatures in EVs are likely to be fragmentation products of the cellular mRNA material, and the extent to which full-length mRNAs are present within EVs remains to be clarified.

Results

Using long-read nanopore RNA sequencing, we sought to characterize the full-length polyadenylated (poly-A) transcriptome of EVs released by human chronic myelogenous leukemia K562 cells. We detected 443 and 280 RNAs that were respectively enriched o

SUBMITTER: Padilla JA 

PROVIDER: S-EPMC10514964 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature