Loop-mediated isothermal amplification identifies nematode Leidynema in the hindgut of non-pest cockroach.

ABSTRACT: Cockroach microbiome studies generally focus on pest cockroach species belonging to the Blattidae and Ectobiidae families. There are no reports characterizing the gut microbiome of non-pest cockroach species Blaberus discoidalis (family Blaberidae), which is commonly used as a food source for insectivorous animals. We discovered the parasitic nematode Leidynema appendiculata in the B. discoidalis hindgut during initial work characterizing the gut microbiome of this organism. To determine the proportion of the B. discoidalis colony that was colonized by L. appendiculata, 28 S rDNA was amplified using two Methods: endpoint polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). B. discoidalis colonies were raised on three diet types (control, high fibre, and high f

SUBMITTER: Schnell LJ 

PROVIDER: S-EPMC10515063 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature