Efficient Agrobacterium-mediated transformation and genome editing of Fagopyrum tataricum.

ABSTRACT: Fagopyrum tataricum (L.) Gaertn. is an exceptional crop known for its remarkable health benefits, high levels of beneficial polyphenols and gluten-free properties, making it highly sought-after as a functional food. Its self-fertilisation capability and adaptability to challenging environments further contribute to its potential as a sustainable agricultural option. To harness its unique traits, genetic transformation in F. tataricum is crucial. In this study, we optimised the Agrobacterium-mediated transformation protocol for F. tataricum callus, resulting in a transformation rate of regenerated plants of approximately 20%. The protocol's effectiveness was confirmed through successful GUS staining, GFP expression, and the generation of albino plants via FtPDS g

SUBMITTER: Pinski A 

PROVIDER: S-EPMC10515086 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature