Anti-SpCas9 IgY Polyclonal Antibodies Production for CRISPR Research Use.

ABSTRACT: The CRISPR/Cas adaptative immune system has been harnessed as an RNA-guided, programmable genome editing tool, allowing for diverse biotechnological applications. The implementation of the system relies on the ability to detect the Cas9 protein in biological samples. This task is facilitated by employing antibodies, which exhibit several advantageous features and applications in the context of tropical neglected diseases. This study reports a one-month immunization scheme with the Cas9 protein fromStreptococcus pyogenes to produce IgY polyclonal antibodies (anti-SpCas9), which can be rapidly isolated by combining yolk de-lipidation with protein salting out using pectin and ammonium sulfate, respectively. Immunodetection assays indicate that the antibodies are highly sensitive, speci

SUBMITTER: Leon E 

PROVIDER: S-EPMC10515394 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature