Lipid peroxidation drives liquid-liquid phase separation and disrupts raft protein partitioning in biological membranes.

ABSTRACT: The peroxidation of membrane lipids by free radicals contributes to aging, numerous diseases, and ferroptosis, an iron-dependent form of cell death. Peroxidation changes the structure, conformation and physicochemical properties of lipids, leading to major membrane alterations including bilayer thinning, altered fluidity, and increased permeability. Whether and how lipid peroxidation impacts the lateral organization of proteins and lipids in biological membranes, however, remains poorly understood. Here, we employ cell-derived giant plasma membrane vesicles (GPMVs) as a model to investigate the impact of lipid peroxidation on ordered membrane domains, often termed membrane rafts. We show that lipid peroxidation induced by the Fenton reaction dramatically enhances phase separation propensit

SUBMITTER: Balakrishnan M 

PROVIDER: S-EPMC10515805 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature