Tissue-Specific Dependence of Th1 Cells on the Amino Acid Transporter SLC38A1 in Inflammation.

ABSTRACT: Amino acid (AA) uptake is essential for T cell metabolism and function, but how tissue sites and inflammation affect CD4⁺ T cell subset requirements for specific AA remains uncertain. Here we tested CD4⁺ T cell AA demands with in vitro and multiple in vivo CRISPR screens and identify subset- and tissue-specific dependencies on the AA transporter SLC38A1 (SNAT1). While dispensable for T cell persistence and expansion over time in vitro and in vivo lung inflammation, SLC38A1 was critical for Th1 but not Th17 cell-driven Experimental Autoimmune Encephalomyelitis (EAE) and contributed to Th1 cell-driven inflammatory bowel disease. SLC38A1 deficiency reduced mTORC1 signaling and glycolytic activity in Th1 cells, in part by reducing intracellular

SUBMITTER: Sugiura A 

PROVIDER: S-EPMC10515961 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature