Single-cell nascent RNA sequencing using click-chemistry unveils coordinated transcription.

ABSTRACT: Transcription is the primary regulatory step in gene expression. Divergent transcription initiation from promoters and enhancers produces stable RNAs from genes and unstable RNAs from enhancers^1-5. Nascent RNA capture and sequencing assays simultaneously measure gene and enhancer activity in cell populations^6-9. However, fundamental questions in the temporal regulation of transcription and enhancer-gene synchrony remain unanswered primarily due to the absence of a single-cell perspective on active transcription. In this study, we present scGRO-seq - a novel single-cell nascent RNA sequencing assay using click-chemistry - and unveil the coordinated transcription throughout the genome. scGRO-seq demonstrates the episodic nature of transcription, and estimates burst size

SUBMITTER: Mahat DB 

PROVIDER: S-EPMC10516050 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature