An antibody-based molecular switch for continuous small-molecule biosensing.

ABSTRACT: We present a generalizable approach for designing biosensors that can continuously detect small-molecule biomarkers in real time and without sample preparation. This is achieved by converting existing antibodies into target-responsive "antibody-switches" that enable continuous optical biosensing. To engineer these switches, antibodies are linked to a molecular competitor through a DNA scaffold, such that competitive target binding induces scaffold switching and fluorescent signaling of changing target concentrations. As a demonstration, we designed antibody-switches that achieve rapid, sample preparation-free sensing of digoxigenin and cortisol in undiluted plasma. We showed that, by substituting the molecular competitor, we can further modulate the sensitivity of our cortisol switch to ac

SUBMITTER: Thompson IAP 

PROVIDER: S-EPMC10516488 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature