Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures.

ABSTRACT: The longstanding model is that most bloodstream infections (BSIs) are caused by a single organism. We perform whole genome sequencing of five-to-ten strains from blood culture (BC) bottles in each of ten patients with Candida glabrata BSI. We demonstrate that BCs contain mixed populations of clonal but genetically diverse strains. Genetically distinct strains from two patients exhibit phenotypes that are potentially important during BSIs, including differences in susceptibility to antifungal agents and phagocytosis. In both patients, the clinical microbiology lab recovered a fluconazole-susceptible index strain, but we identify mixed fluconazole-susceptible and -resistant populations. Diversity in drug susceptibility is likely clinically relevant, as fluconazole-resistant strains were subs

SUBMITTER: Badrane H 

PROVIDER: S-EPMC10516878 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature