Electric field stimulation unmasks a subtle role for T-type calcium channels in regulating lymphatic contraction.

ABSTRACT: We previously identified two isoforms of T-type, voltage-gated calcium (Ca_v3) channels (Ca_v3.1, Ca_v3.2) that are functionally expressed in murine lymphatic muscle cells; however, contractile tests of lymphatic vessels from single and double Ca_v3 knock-out (DKO) mice, exhibited nearly identical parameters of spontaneous twitch contractions as wild-type (WT) vessels, suggesting that Ca_v3 channels play no significant role. Here, we considered the possibility that the contribution of Ca_v3 channels might be too subtle to detect in standard contraction analyses. We compared the sensitivity of lymphatic vessels from WT and Ca_v3 DKO mice to the L-type calcium channel (Ca_v1.2) inhibitor nifedipine and found that

SUBMITTER: Davis MJ 

PROVIDER: S-EPMC10516884 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature