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Structural and functional characterization of USP47 reveals a hot spot for inhibitor design.


ABSTRACT: USP47 is widely involved in tumor development, metastasis, and other processes while performing a more regulatory role in inflammatory responses, myocardial infarction, and neuronal development. In this study, we investigate the functional and biochemical properties of USP47, whereby depleting USP47 inhibited cancer cell growth in a p53-dependent manner-a phenomenon that enhances during the simultaneous knockdown of USP7. Full-length USP47 shows higher deubiquitinase activity than the catalytic domain. The crystal structures of the catalytic domain, in its free and ubiquitin-bound states, reveal that the misaligned catalytic triads, ultimately, become aligned upon ubiquitin-binding, similar to USP7, thereby becoming ready for catalysis. Yet, the composition and lengths of BL1, BL2, and BL3 of USP47 differ from those for USP7, and they contribute to the observed selectivity. Our study provides molecular details of USP47 regulation, substrate recognition, and the hotspots for drug discovery by targeting USP47.

SUBMITTER: Shin SC 

PROVIDER: S-EPMC10516900 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Structural and functional characterization of USP47 reveals a hot spot for inhibitor design.

Shin Sang Chul SC   Park Jinyoung J   Kim Kyung Hee KH   Yoon Jung Min JM   Cho Jinhong J   Ha Byung Hak BH   Oh Yeonji Y   Choo Hyunah H   Song Eun Joo EJ   Kim Eunice EunKyeong EE  

Communications biology 20230922 1


USP47 is widely involved in tumor development, metastasis, and other processes while performing a more regulatory role in inflammatory responses, myocardial infarction, and neuronal development. In this study, we investigate the functional and biochemical properties of USP47, whereby depleting USP47 inhibited cancer cell growth in a p53-dependent manner-a phenomenon that enhances during the simultaneous knockdown of USP7. Full-length USP47 shows higher deubiquitinase activity than the catalytic  ...[more]

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