High-level in vitro resistance to gentamicin acquired in a stepwise manner in Neisseria gonorrhoeae.

ABSTRACT:

Objectives

Gentamicin is used in several alternative treatments for gonorrhoea. Verified clinical Neisseria gonorrhoeae isolates with gentamicin resistance are mainly lacking and understanding the mechanisms for gonococcal gentamicin resistance is imperative. We selected gentamicin resistance in gonococci in vitro, identified the novel gentamicin-resistance mutations, and examined the biofitness of a high-level gentamicin-resistant mutant.

Methods

Low- and high-level gentamicin resistance was selected in WHO X (gentamicin MIC = 4 mg/L) on gentamicin-gradient agar plates. Selected mutants were whole-genome sequenced. Potential gentamicin-resistance fusA mutations were transformed into WT strains to verify their impact on gentamicin MICs. The biofitness of high-level gentamici

SUBMITTER: Golparian D 

PROVIDER: S-EPMC10517096 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature