Exploration of cell state heterogeneity using single-cell proteomics through sensitivity-tailored data-independent acquisition.

ABSTRACT: Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-state heterogeneity and distinct cellular signaling patterns that remain obscured with population-based techniques. The limited amount of material encapsulated in a single cell however, raises significant technical challenges to molecular profiling. Due to extensive optimization efforts, single-cell proteomics by Mass Spectrometry (scp-MS) has emerged as a powerful tool to facilitate proteome profiling from ultra-low amounts of input, although further development is needed to realize its full potential. To this end, we carry out comprehensive analysis of orbitrap-based data-independent acquisition (DIA) for limited material proteomics. Notably, we find a fundamental difference between optimal DIA m

SUBMITTER: Petrosius V 

PROVIDER: S-EPMC10517177 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature