Rice transformation treatments leave specific epigenome changes beyond tissue culture.

ABSTRACT: During transgenic plant production, tissue culture often carries epigenetic, and genetic changes that underlie somaclonal variations, leading to unpredictable phenotypes. Additionally, specific treatments for rice (Oryza sativa) transformation processes may individually or jointly contribute to somaclonal variations, but their specific impacts on rice epigenomes toward transcriptional variations remain unknown. Here, the impact of individual transformation treatments on genome-wide DNA methylation and the transcriptome were examined. In addition to activating stress-responsive genes, individual transformation components targeted different gene expression modules that were enriched in specific functional categories. The transformation treatments strongly impacted DNA methylation and express

SUBMITTER: Hsieh JA 

PROVIDER: S-EPMC10517251 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature