Improved Production of Induced Pluripotent Stem Cells Using Dot Pattern Culture Plates.

ABSTRACT: The rate of cell proliferation is a crucial factor in cell production under good manufacturing practice (GMP) control. In this study, we identified a culture system for induced pluripotent cells (iPSCs) that supports cell proliferation and viability and maintains the cells in an undifferentiated state even at 8 days after seeding. This system involves the use of dot pattern culture plates that have been coated with a chemically defined scaffold which has high biocompatibility. Under cell starvation conditions, where medium exchange was not performed for 7 days or where the amount of medium exchange was reduced to half or a quarter, iPSC viability and lack of differentiation were maintained. The rate of cell viability in this culture system was greater than generally obtained by standard cu

SUBMITTER: Nakashima Y 

PROVIDER: S-EPMC10517333 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature