Panobinostat (LBH589) combined with AM1241 induces cervical cancer cell apoptosis through autophagy pathway.

ABSTRACT:

Purpose

The study aims to investigate the apoptotic effects of combining LBH589 and AM1241 (a selective CB2 receptor agonist) on cervical cancer cells and elucidating the mechanism of this combined therapy, which may provide innovative strategies for treating this disease.

Methods

The viability of the cervical cancer cells was measured by cell counting kit-8 (CCK-8) assay, and the synergistic effect was analyzed using SynergyFinder. Cell proliferation was tested by cell cloning. The apoptosis and reactive oxygen species (ROS) production in cervical cancer cells were analyzed by flow cytometry. Western blot and quantitative real-time PCR (qRT-PCR) were employed to determine changes in protein and gene levels of pathway-related factors.

Results

By the results of cytotox

SUBMITTER: Sheng B 

PROVIDER: S-EPMC10517494 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature