Dynamic Data-Independent Acquisition Mass Spectrometry with Real-Time Retrospective Alignment.

ABSTRACT: Data-independent acquisition (DIA) mass spectrometry has grown in popularity in recent years, because of the reproducibility and quantitative rigor of a systematic tandem mass spectrometry (MS/MS) sampling method. However, traditional DIA methods may spend valuable instrument time acquiring MS/MS spectra with no usable information in them, affecting sensitivity and quantitative performance. We developed a DIA strategy that dynamically adjusts the MS/MS windows during the chromatographic separation. The method focuses MS/MS acquisition on the most relevant mass range at each point in time─increasing the quantitative sensitivity by increasing the time spent on each DIA window. We demonstrate an improved lower limit of quantification, on average, without sacrificing the number of peptides det

SUBMITTER: Heil LR 

PROVIDER: S-EPMC10517878 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature