Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.

ABSTRACT: Adult hippocampal neurogenesis (AHN) gives rise to new neurons throughout life. This phenomenon takes place in more than 120 mammalian species, including humans, yet its occurrence in the latter was questioned after one study proposed the putative absence of neurogenesis markers in the adult human hippocampus. In this regard, we showed that prolonged fixation impedes the visualization of Doublecortin⁺ immature neurons in this structure, whereas other authors have suggested that a dilated post-mortem delay (PMD) underlies these discrepancies. Nevertheless, the individual and/or additive contribution of fixation and the PMD to the detection (or lack thereof) of other AHN markers has not been studied to date. To address this pivotal question, we used a tightly controlled experiment

SUBMITTER: Gallardo-Caballero M 

PROVIDER: S-EPMC10517969 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature