Identification of a Bile Acid-Binding Transcription Factor in Clostridioides difficile Using Chemical Proteomics.

ABSTRACT: Clostridioides difficile is a Gram-positive anaerobic bacterium that is the leading cause of hospital-acquired gastroenteritis in the US. In the gut milieu, C. difficile encounters microbiota-derived, growth-inhibiting bile acids that are thought to be a significant mechanism of colonization resistance. While the levels of certain bile acids in the gut correlate with susceptibility to C. difficile infection, their molecular targets in C. difficile remain unknown. In this study, we sought to use chemical proteomics to identify bile acid-interacting proteins in C. difficile. Using photoaffinity bile acid probes and chemical proteomics, we identified a previously uncharacterized MerR family protein, CD3583 (now BapR), as a putative bile acid-sensing transcri

SUBMITTER: Forster ER 

PROVIDER: S-EPMC10518218 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature