Persister cell phenotypes contribute to poor patient outcomes after neoadjuvant chemotherapy in PDAC.

ABSTRACT: Neoadjuvant chemotherapy can improve the survival of individuals with borderline and unresectable pancreatic ductal adenocarcinoma; however, heterogeneous responses to chemotherapy remain a significant clinical challenge. Here, we performed RNA sequencing (n = 97) and multiplexed immunofluorescence (n = 122) on chemo-naive and postchemotherapy (post-CTX) resected patient samples (chemoradiotherapy excluded) to define the impact of neoadjuvant chemotherapy. Transcriptome analysis combined with high-resolution mapping of whole-tissue sections identified GATA6 (classical), KRT17 (basal-like) and cytochrome P450 3A (CYP3A) coexpressing cells that were preferentially enriched in post-CTX resected samples. The persistence of GATA6^hi and KRT17^hi cells post-CTX was significan

SUBMITTER: Zhou X 

PROVIDER: S-EPMC10518256 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature