A database of restriction maps to expand the utility of bacterial artificial chromosomes.

ABSTRACT: While Bacterial Artificial Chromosomes libraries were once a key resource for the genomic community, they have been obviated, for sequencing purposes, by long-read technologies. Such libraries may now serve as a valuable resource for manipulating and assembling large genomic constructs. To enhance accessibility and comparison, we have developed a BAC restriction map database. Using information from the National Center for Biotechnology Information's cloneDB FTP site, we constructed a database containing the restriction maps for both uniquely placed and insert-sequenced BACs from 11 libraries covering the recognition sequences of the available restriction enzymes. Along with the database, we generated a set of Python functions to reconstruct the database and more easily access the informati

SUBMITTER: Winden E 

PROVIDER: S-EPMC10518450 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature