A basic phosphoproteomic-DIA workflow integrating precise quantification of phosphosites in systems biology.

ABSTRACT: Phosphorylation is one of the most important post-translational modifications (PTMs) of proteins, governing critical protein functions. Most human proteins have been shown to undergo phosphorylation, and phosphoproteomic studies have been widely applied due to recent advancements in high-resolution mass spectrometry technology. Although the experimental workflow for phosphoproteomics has been well-established, it would be useful to optimize and summarize a detailed, feasible protocol that combines phosphoproteomics and data-independent acquisition (DIA), along with follow-up data analysis procedures due to the recent instrumental and bioinformatic advances in measuring and understanding tens of thousands of site-specific phosphorylation events in a single experiment. Here, we describe an o

SUBMITTER: Di Y 

PROVIDER: S-EPMC10518521 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature