A hydrogen-deuterium exchange mass spectrometry-based protocol for protein-small molecule interaction analysis.

ABSTRACT: Protein-small molecule interaction is vital in regulating protein functions and controlling various cellular processes. Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful methodology to study protein-small molecule interactions, however, to accurately probe the conformational dynamics of the protein upon small molecule binding, the HDX-MS experimental conditions should be carefully controlled and optimized. Here, we present the detailed continuous-labeling, bottom-up HDX-MS protocol for studying protein-small molecule interactions. We took a side-by-side HDX kinetics comparison of the Hsp90N protein with or without the treatment of small molecules (i.e., Radicicol, Geldanamycin) for displaying conformational changes induced by molecular interactions between

SUBMITTER: Meng Q 

PROVIDER: S-EPMC10518522 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature