The effect of PACAP administration on LPS-induced cytokine expression in the Atlantic salmon SHK-1 cell line.

ABSTRACT: Recent work has identified pituitary adenylate cyclase activating polypeptide (PACAP) as a potential antimicrobial and immune stimulating agent which may be suitable for use in aquaculture. However, its effects on teleost immunity are not well studied and may be significantly different than what has been observed in mammals. In this study we examined the effects of PACAP on the Atlantic salmon macrophage cell line SHK-1. PACAP was able to increase the expression of LPS-induced il-1β in at concentrations of 1 uM when administered 24h prior to LPS stimulation. Furthermore, concentrations as low as 40nM had an effect when administered both 24h prior and in tandem with LPS. PACAP was also capable of increasing the expression of il-1β and tnf-α in SHK-1 cells challenged with a low dose of heat-

SUBMITTER: Campbell JH 

PROVIDER: S-EPMC10518582 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature