TPN-COF@Fe-MIL-100 composite used as an electrochemical aptasensor for detection of trace tetracycline residues.

ABSTRACT: In this work, a metal-organic framework@covalent organic framework composite (TPN-COF@Fe-MIL-100) was prepared and used as a sensing material to construct an aptasensor for trace detection of tetracycline (TET). The TPN-COF@Fe-MIL-100 integrates a large surface area, porous structure, excellent electrochemical activity, rich chemical functionality, and strong bioaffinity for aptamers, providing abundant active sites to effectively anchor aptamer strands. As a result, the TPN-COF@Fe-MIL-100-based aptasensor shows high sensitivity for detecting TET via specific recognition between aptamer and TET to form G-quadruplex. An ultralow detection limit of 1.227 fg mL^-1 is deduced from the electrochemical impedance spectroscopy within a wide linear range of 0.01-10000 pg mL^-1 for TET. The TPN-COF@Fe-MIL-100-based aptasensor also exhibits good selectivity, reproducibility, stability, regenerability, and applicability for a real milk sample. Therefore, the TPN-COF@Fe-MIL-100-based aptasensor will be promising for detecting trace harmful antibiotics residues for food safety.

SUBMITTER: Meng Y 

PROVIDER: S-EPMC10518659 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature