Dataset Information

Adjustable Fluorescence Emission of J-Aggregated Tricarbocyanine in the Near-Infrared-II Region.

ABSTRACT: Near-infrared (NIR) J-aggregates attract increasing attention in many areas, especially in biomedical applications, as they combine the advantages of NIR spectroscopy with the unique J-aggregation properties of organic dyes. They enhance light absorption and have been used as effective biological imaging and therapeutic agents to achieve high-resolution imaging or effective phototherapy in vivo. In this work, we present novel J-aggregates composed of the well-known cyanine molecules. Cyanines are one of the few types of molecules whose absorption and emission can be shifted over a broad spectral range, from the ultraviolet (UV) to the NIR regime. They can easily transform into J-aggregates with narrow absorption and emission peaks, which is accompanied by a red shift in their spectra. In this work, we show, for the first time, that the tricarbocyanine dye (IR 820) has two sharp fluorescence emission bands in the NIR-II region with high photostability. These emission bands can be tuned to a desired wavelength in the range of 1150-1560 and 1675 nm, with a linear dependence on the excitation wavelength. Cryogenic transmission electron microscopy (cryo-TEM) images are presented, and combined with molecular modeling analysis, they confirm IR 820 π-stacked self-assembled fibrous structures.

SUBMITTER: Dar N

PROVIDER: S-EPMC10518818 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Publications

Adjustable Fluorescence Emission of J-Aggregated Tricarbocyanine in the Near-Infrared-II Region.

Dar Nitzan N Weissman Haim H Ankri Rinat R

The journal of physical chemistry. B 20230908 37

Near-infrared (NIR) J-aggregates attract increasing attention in many areas, especially in biomedical applications, as they combine the advantages of NIR spectroscopy with the unique J-aggregation properties of organic dyes. They enhance light absorption and have been used as effective biological imaging and therapeutic agents to achieve high-resolution imaging or effective phototherapy in vivo. In this work, we present novel J-aggregates composed of the well-known cyanine molecules. Cyanines ar ...[more]

PMID: 37682586

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Project description:The primary treatment for malignant tumors remains to be resection. The strongest predictor of recurrence and postoperative prognosis is whether diseased tissue/cells remain(s) at the surgical margin. Cancer surgery entails surgeons having the capability to visually distinguish between subtle shades of color in attempts of differentiating between diseased tissue and healthy tissue under standard white-light illumination, as such tissue states appear identical at the meso-/macroscopic level. Accordingly, enhancing the capability of surgeons to do so such that they can accurately delineate the tumor margin is of paramount importance. Fluorescence-guided surgery facilitates in enhancing such capability by color-coding the surgical field with overlaid contrasting pseudo-colors from real-time intraoperative fluorescence emission via utilizing fluorescent constructs in tandem. Constructs undergoing clinical trials or that are FDA-approved provide peak fluorescence emission in the visible (405 - 700 nm) or near-infrared-I (NIR-I) spectral region (700-900 nm), whereby differentiation between tissue states progressively improves in sync with using constructs that emit longer wavelengths of light. Here, we repurpose the usage of such fluorescent constructs by establishing feasibility of a tumor-targeting immunoconjugate (cetuximab-IRDye800) having peak fluorescence emission at the NIR-I spectral region to provide improved tumor margin delineation by affording higher tumor-to-background ratios (TBRs) when measuring its off-peak fluorescence emission at the near-infrared-II (NIR-II) spectral region (1000-1700 nm) in in vivo applications. We prepared murine tumor models, administered such immunoconjugate, and imaged such models pre-/post-administration via utilizing imaging systems that separately afforded acquisition of fluorescence emission in the NIR-I or NIR-II spectral region. On doing so, we determined in vivo TBRs, ex vivo TBRs with/-out skin, and ex vivo biodistribution, all via measuring the fluorescence emission of the immunoconjugate at tumor site(s) at both spectral regions. Collectively, we established feasibility of using the immunoconjugate to afford improved tumor margin delineation by providing 2-fold higher TBRs via utilizing the NIR-II spectral region to capture off-peak fluorescence emission from a fluorescent construct having NIR-I peak fluorescence emission.

Dataset Information

Adjustable Fluorescence Emission of J-Aggregated Tricarbocyanine in the Near-Infrared-II Region.

Publications

Adjustable Fluorescence Emission of J-Aggregated Tricarbocyanine in the Near-Infrared-II Region.

Similar Datasets

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